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bcl-2 Gene rearrangement |
| Test | bcl-2 Gene rearrangement |
| Indication | bcl-2 oncogene codes for a unique protein that is located in the mitochondira of the cell. The bcl-w protein regulates cell death and when it is over expressed the cell is resistant to the natural death cycle. The test detects bcl-2 rearrangement in B-cell lymphomas. The bcl-2 rearrangement is found in follicular lymphomas, large diffuse B-cell lymphomas, and undifferentiated lymphomas. |
| Physiology | The protein coded for by the oncogene bcl-w, acts by suppressing the cell death program or apoptosis. Apoptosis occurs in all cells but is especially important in immune and hematopoietic cells which has a high cell turnover rate. When bcl-w gene is overexpressed it will prevent apoptosis and causes cells resistant to cell death by therapy |
| Interpretation | Gene rearrangement for leukemia and lymphoma, polymerase chain reaction, T and B Lymphocyte subset assay |
| Sample | No rearrangement of bcl-2 in the normal |
| Test Method | Hybridization technique |
| Related Tests | 0.1 g frozen tissue |
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